Anti-CD15 antibody [28] (ab20137)
Key features and details
- Mouse monoclonal [28] to CD15
- Suitable for: IP, Flow Cyt, IHC-Fr
- Isotype: IgM
Overview
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Product name
Anti-CD15 antibody [28]
See all CD15 primary antibodies -
Description
Mouse monoclonal [28] to CD15 -
Host species
Mouse -
Specificity
Specific to CD15. 28 is useful for leukaemia typing and purification of stem cells. -
Tested applications
Suitable for: IP, Flow Cyt, IHC-Frmore details -
Species reactivity
Predicted to work with: Human -
Immunogen
Tissue, cells or virus corresponding to CD15. Monocytes, separated from other peripheral blood leucocytes (PBL) on fibronectin plates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
28 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr |
Use at an assay dependent concentration.
Note: samples should be acetone fixed. |
Notes |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. Note: samples should be acetone fixed. |
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab20137 has been referenced in 5 publications.
- Puttipanyalears C et al. Quantitative STAU2 measurement in lymphocytes for breast cancer risk assessment. Sci Rep 11:915 (2021). PubMed: 33441653
- Liu J et al. Co-expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis. Int J Mol Med 43:1687-1698 (2019). PubMed: 30816446
- Donovan LK et al. A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles. Transl Oncol 5:141-54 (2012). ICC/IF, Flow Cyt ; Human . PubMed: 22741033
- Hogg N et al. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology 53:753-67 (1984). PubMed: 6389324
- Linch DC et al. Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML. Blood 63:566-73 (1984). PubMed: 6365201