The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesCharacterization of leukemias in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
CD15 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD15 is sufficient for labelling of 1x106 cells.
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis.
Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.