Overview

  • Product nameAnti-CD15 antibody [MC-1]See all CD15 primary antibodies ...
  • Description
    Mouse monoclonal [MC-1] to CD15
  • SpecificityDetects the CD15 antigen (Mw 150kDa, 95kDa) found on neutrophils, eosinophils and moncytes. Reed Sternberg cells in Hodgins lymphoma are positive with this antibody.
  • Tested applicationsIHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • General notesThis antibody has the ability to fix complement.

Properties

Applications

Our Abpromise guarantee covers the use of ab6414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Flow Cyt
  • Application notesFlow Cyt: 1/300.
    IHC-P: 1/200 - 1/400.
    Samples may require neuraminidase treatment to reveal the antigen.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
    • Alternative names
      • 3-FAL antibody
      • Alpha 13 fucosyltransferase FucT antibody
      • Alpha-(1,3)-fucosyltransferase antibody
      • ELAM 1 ligand antibody
      • ELAM ligand fucosyltransferase antibody
      • ELAM-1 ligand fucosyltransferase antibody
      • ELFT antibody
      • FCT3A antibody
      • Fuc-TIV antibody
      • Fucosyltransferase 4 alpha 1 3 fucosyltransferase myeloid specific antibody
      • Fucosyltransferase 4 antibody
      • Fucosyltransferase antibody
      • Fucosyltransferase IV antibody
      • FucT-IV antibody
      • FUCTIV antibody
      • FUT4 antibody
      • FUT4_HUMAN antibody
      • Galactoside 3 L fucosyltransferase antibody
      • Galactoside 3-L-fucosyltransferase antibody
      • Leu M1 antibody
      see all

    Anti-CD15 antibody [MC-1] images

    • ab6414 (1µg/ml) staining CD15 in granulocytes of human myometrium.
      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References for Anti-CD15 antibody [MC-1] (ab6414)

    ab6414 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"