Mouse monoclonal [MCS-1] to CD15 (Allophycocyanin)
ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
Specificityab91151 recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. It reacts with the promyelocytes, myelocytes and
polymorphonuclear cells. It specifically labels neutrophils, eosinophils and monoblastoid precursor cells of the myeloid lineage. ab91151 does not react with platelets and cells of the T and B lymphocyte lineage.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 20 µl for 106 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis.
Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.