SpecificityThis antibody is specific to CD15 antigen (Lewisx blood group antigen). We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. ab18396-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis.
Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.