Overview

  • Product nameAnti-CD15 antibody [MY-1]
    See all CD15 primary antibodies
  • Description
    Mouse monoclonal [MY-1] to CD15
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified neutrophils from normal human peripheral blood.

  • EpitopeThis antibody recognizes an epitope involving the carbohydrate sequence 3 fucosyl N acetyllacetosamine.
  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
WB
  • Application notesImmunohistochemistry: 1 / 10 - 1 / 25 for 30 - 60 mins at RT (ABC method).
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.
  • Target

    • RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
    • Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
    • Database links
    • Alternative names
      • 3-FAL antibody
      • Alpha-3-fucosyltransferase antibody
      • ELAM 1 ligand antibody
      • ELAM ligand fucosyltransferase antibody
      • ELAM-1 ligand fucosyltransferase antibody
      • ELFT antibody
      • FCT3A antibody
      • FUC-TIV antibody
      • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) antibody
      • Fucosyltransferase antibody
      • fucosyltransferase IV antibody
      • fucT-IV antibody
      • FUTIV antibody
      • galactoside 3-L-fucosyltransferase antibody
      • Leu M1 antibody
      • Lewis X antibody
      • LeX antibody
      • SSEA-1 antibody
      • stage-specific embryonic antigen 1 antibody
      see all

    Anti-CD15 antibody [MY-1] images

    • ab754 at a dilution of 1/100 staining CD15 from human bronchus sections by Immunohistochemistry (Paraffin-embedded sections). The antibody was incubated with the tissue for 2 hours and then detected with an Alexa-Fluor®  488 conjugated anti-mouse IgM antibody.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

      See Abreview

    References for Anti-CD15 antibody [MY-1] (ab754)

    This product has been referenced in:
    • Fernandez-L A  et al. YAP1 is amplified and up-regulated in hedgehog-associated medulloblastomas and mediates Sonic hedgehog-driven neural precursor proliferation. Genes Dev 23:2729-41 (2009). IHC-P ; Mouse . Read more (PubMed: 19952108) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Sample Mouse Tissue lysate - whole (Mouse brain)
    Specification Mouse brain
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2014

    Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.

    Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some ant...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human Tonsil)
    Specification Human Tonsil
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jul 02 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Spleen)
    Specification Spleen
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 22 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (bronchus)
    Specification bronchus
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"