Overview

  • Product name
    Anti-CD15 antibody [MY-1]
    See all CD15 primary antibodies
  • Description
    Mouse monoclonal [MY-1] to CD15
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified neutrophils from normal human peripheral blood.

  • Epitope
    This antibody recognizes an epitope involving the carbohydrate sequence 3 fucosyl N acetyllacetosamine.
  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
1/10 - 1/25 for 30 - 60 mins at RT (ABC method).

 

IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization
    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Database links
  • Alternative names
    • 3-FAL antibody
    • Alpha-3-fucosyltransferase antibody
    • ELAM 1 ligand antibody
    • ELAM ligand fucosyltransferase antibody
    • ELAM-1 ligand fucosyltransferase antibody
    • ELFT antibody
    • FCT3A antibody
    • FUC-TIV antibody
    • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) antibody
    • Fucosyltransferase antibody
    • fucosyltransferase IV antibody
    • fucT-IV antibody
    • FUTIV antibody
    • galactoside 3-L-fucosyltransferase antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • ab754 at a dilution of 1/100 staining CD15 from human bronchus sections by Immunohistochemistry (Paraffin-embedded sections). The antibody was incubated with the tissue for 2 hours and then detected with an Alexa-Fluor®  488 conjugated anti-mouse IgM antibody.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

    See Abreview

References

This product has been referenced in:
  • Ruhland MK  et al. Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis. Nat Commun 7:11762 (2016). Read more (PubMed: 27272654) »
  • Han S  et al. Pre-treatment neutrophil-to-lymphocyte ratio is associated with neutrophil and T-cell infiltration and predicts clinical outcome in patients with glioblastoma. BMC Cancer 15:617 (2015). IHC . Read more (PubMed: 26341881) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lung)
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde
Username

Jim Hsiao

Verified customer

Submitted Feb 05 2018

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Mouse brain)
Specification
Mouse brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 09 2014

Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.

Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some ant...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Tonsil)
Specification
Human Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jul 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bronchus)
Specification
bronchus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
Username

Abcam user community

Verified customer

Submitted Dec 12 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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