Overview

  • Product name
    Anti-CD15 antibody [MY-1]
    See all CD15 primary antibodies
  • Description
    Mouse monoclonal [MY-1] to CD15
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified neutrophils from normal human peripheral blood.

  • Epitope
    This antibody recognizes an epitope involving the carbohydrate sequence 3 fucosyl N acetyllacetosamine.
  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
WB
  • Application notes
    Immunohistochemistry: 1 / 10 - 1 / 25 for 30 - 60 mins at RT (ABC method).
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.
  • Target

    • Relevance
      CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
    • Cellular localization
      Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
    • Database links
    • Alternative names
      • 3-FAL antibody
      • Alpha-3-fucosyltransferase antibody
      • ELAM 1 ligand antibody
      • ELAM ligand fucosyltransferase antibody
      • ELAM-1 ligand fucosyltransferase antibody
      • ELFT antibody
      • FCT3A antibody
      • FUC-TIV antibody
      • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) antibody
      • Fucosyltransferase antibody
      • fucosyltransferase IV antibody
      • fucT-IV antibody
      • FUTIV antibody
      • galactoside 3-L-fucosyltransferase antibody
      • Leu M1 antibody
      • Lewis X antibody
      • LeX antibody
      • SSEA-1 antibody
      • stage-specific embryonic antigen 1 antibody
      see all

    Images

    • ab754 at a dilution of 1/100 staining CD15 from human bronchus sections by Immunohistochemistry (Paraffin-embedded sections). The antibody was incubated with the tissue for 2 hours and then detected with an Alexa-Fluor®  488 conjugated anti-mouse IgM antibody.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

      See Abreview

    References

    This product has been referenced in:
    • Ruhland MK  et al. Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis. Nat Commun 7:11762 (2016). Read more (PubMed: 27272654) »
    • Han S  et al. Pre-treatment neutrophil-to-lymphocyte ratio is associated with neutrophil and T-cell infiltration and predicts clinical outcome in patients with glioblastoma. BMC Cancer 15:617 (2015). IHC . Read more (PubMed: 26341881) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Sample
    Mouse Tissue lysate - whole (Mouse brain)
    Specification
    Mouse brain
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2014

    Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.

    Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some ant...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Tonsil)
    Specification
    Human Tonsil
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jul 02 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 22 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (bronchus)
    Specification
    bronchus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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