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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab33315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.|
|Flow Cyt||1/50 - 1/100.
1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
|ICC/IF||Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr.|
|ELISA||Use at an assay dependent concentration.|
ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.