Overview

  • Product nameAnti-CD151 antibody [11G5a]
    See all CD151 primary antibodies
  • Description
    Mouse monoclonal [11G5a] to CD151
  • Specificityab33315 recognises the human CD151 cell surface antigen, also known as PETA-3.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, IP, WB, Flow Cyt, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Tonsil/spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab33315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
Flow Cyt 1/50 - 1/100.

1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr.
ELISA Use at an assay dependent concentration.

Target

  • FunctionEssential for the proper assembly of the glomerular and tubular basement membranes in kidney.
  • Tissue specificityExpressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
  • Involvement in diseaseDefects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD151 antibody
    • CD151 antigen antibody
    • CD151 molecule (Raph blood group) antibody
    • CD151 molecule antibody
    • CD151_HUMAN antibody
    • GP27 antibody
    • hemidesmosomal tetraspanin CD151 antibody
    • Membrane glycoprotein SFA-1 antibody
    • MER2 antibody
    • PETA-3 antibody
    • PETA3 antibody
    • Platelet endothelial tetraspan antigen 3 antibody
    • platelet surface glycoprotein gp27 antibody
    • Platelet-endothelial cell tetraspan antigen 3 antibody
    • Platelet-endothelial tetraspan antigen 3 antibody
    • RAPH antibody
    • Red blood cell antigen MER2 antibody
    • SFA 1 antibody
    • SFA1 antibody
    • Tetraspanin-24 antibody
    • Tspan-24 antibody
    • TSPAN24 antibody
    see all

Anti-CD151 antibody [11G5a] images

  • ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.  

    See Abreview

  • ICC/IF image of ab33315 stained HT 1080 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33315, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96789, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CD151 antibody [11G5a] (ab33315)

This product has been referenced in:
  • Palmer TD  et al. Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering and is a clinical indicator of prostate cancer progression. Cancer Res 74:173-87 (2014). Read more (PubMed: 24220242) »
  • Matsumoto N  et al. Role of CD151 expression in gallbladder carcinoma. Surgery 156:1212-7 (2014). IHC-P ; Human . Read more (PubMed: 24890568) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080)
Loading amount 100000 cells
Specification HT1080
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted May 12 2012

Thank you for contacting us. The antigen retrieval step would be heat-mediated with citrate buffer (pH6.0). Details can be found here: Heat-induced epitope retrieval (HIER) Heat-mediated antigen retrieval may be used in ma...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Fibrosarcoma)
Specification Fibrosarcoma
Preparation Cell harvesting/tissue preparation method: Cells lifted off plastic in PBS/EDTA
Sample buffer: PBS/EDTA
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy viable cells
Username

Abcam user community

Verified customer

Submitted Oct 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Fibrosarcoma HT1080)
Specification Fibrosarcoma HT1080
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy no gate
Username

Abcam user community

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization No
Blocking step Image FX signal enhancer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"