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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab33315 in the following tested applications.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.|
|Flow Cyt||1/50 - 1/100.
1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
|ICC/IF||Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr.|
|ELISA||Use at an assay dependent concentration.|
ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.