The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
Involvement in disease
Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
Karamatic Crew V et al. CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin. Blood104:2217-23 (2004).
Read more (PubMed: 15265795) »