Overview

  • Product name
    Anti-CD151 antibody [B-E31]
    See all CD151 primary antibodies
  • Description
    Mouse monoclonal [B-E31] to CD151
  • Specificity
    This antibody is specific for CD151.
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Eahy 926 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab27406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
  • Tissue specificity
    Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
  • Involvement in disease
    Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD151 antibody
    • CD151 antigen antibody
    • CD151 molecule (Raph blood group) antibody
    • CD151 molecule antibody
    • CD151_HUMAN antibody
    • GP27 antibody
    • hemidesmosomal tetraspanin CD151 antibody
    • Membrane glycoprotein SFA-1 antibody
    • MER2 antibody
    • PETA-3 antibody
    • PETA3 antibody
    • Platelet endothelial tetraspan antigen 3 antibody
    • platelet surface glycoprotein gp27 antibody
    • Platelet-endothelial cell tetraspan antigen 3 antibody
    • Platelet-endothelial tetraspan antigen 3 antibody
    • RAPH antibody
    • Red blood cell antigen MER2 antibody
    • SFA 1 antibody
    • SFA1 antibody
    • Tetraspanin-24 antibody
    • Tspan-24 antibody
    • TSPAN24 antibody
    see all

Images

  • Ab27406, staining CD151 by FACS.
  • ICC/IF image of ab27406 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27406, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab27406 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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