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Our Abpromise guarantee covers the use of ab89196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CD153 with ab89196. Tissue was fixed with 4% PFA at 4C for overnight. No permeabilization. Treated with 20µg/ml Protenase K room tepmperature 15 mins for antigen retrieval. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
ab89196 has not yet been referenced specifically in any publications.