PE Anti-CD16 antibody [3G8] (ab117117)
Key features and details
- PE Mouse monoclonal [3G8] to CD16
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD16 antibody [3G8]
See all CD16 primary antibodies -
Description
PE Mouse monoclonal [3G8] to CD16 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD16. Human neutrophils.
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Positive control
- Flow Cyt: Lymphocytes from human blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
3G8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD16 antibody [3G8] (ab130761)
- PerCP/Cy5.5® Anti-CD16 antibody [3G8], prediluted (ab157322)
- Anti-CD16 antibody [3G8] - Low endotoxin, Azide free (ab176528)
- Alexa Fluor® 488 Anti-CD16 antibody [3G8] (ab187585)
- APC/Cy7® Anti-CD16 antibody [3G8], prediluted (ab197141)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab234209)
- FITC Anti-CD16 antibody [3G8] (ab239254)
- Alexa Fluor® 647 Anti-CD16 antibody [3G8] (ab239275)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- Omim: 146740 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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Alternative names
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
Images
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Flow cytometry multicolor surface staining pattern of human lymphocytes using ab117117 at (20 µL reagent/100 µL of peripheral whole blood) and anti-human CD3 APC antibody at (10 µL reagent/100 µL of peripheral whole blood).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab117117 at (20 µL reagent/100 µL of peripheral whole blood).
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Separation of human CD3 negative CD16 positive NK cells (red-filled) from human CD3 positive CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab117117 at (20 µL reagent/100 µL of peripheral whole blood).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab117117 has been referenced in 1 publication.
- Congy-Jolivet N et al. Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res 68:976-80 (2008). PubMed: 18281470