Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab1254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesEnumeration of NK-cell numbers in human lysed whole peripheral blood or mononuclear cells separated by density gradient.

    CD16 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD16 is sufficient for labelling of 1x106 cells.
  • Target

    • FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
    • Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
    • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Glycosylated. Contains high mannose- and complex-type oligosaccharides.
      The soluble form is produced by a proteolytic cleavage.
    • Cellular localizationCell membrane. Secreted. Exists also as a soluble receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 16 antibody
      • CD 16a antibody
      • CD16 antibody
      • CD16a antibody
      • CD16a antigen antibody
      • CD16B antibody
      • CD16b antigen antibody
      • Fc fragment of IgG antibody
      • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
      • Fc fragment of IgG low affinity IIIa receptor antibody
      • Fc fragment of IgG receptor IIIa antibody
      • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor for antibody
      • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
      • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
      • Fc gamma R3 antibody
      • Fc gamma receptor III 2 (CD 16) antibody
      • Fc gamma receptor III A antibody
      • Fc gamma receptor IIIA antibody
      • Fc gamma receptor IIIb (CD 16) antibody
      • Fc gamma RIII alpha antibody
      • Fc gamma RIII antibody
      • Fc gamma RIII beta antibody
      • Fc gamma RIIIa antibody
      • Fc gamma RIIIb antibody
      • Fc of IgG antibody
      • Fc-gamma receptor III2 (CD 16) antibody
      • Fc-gamma receptor III2 (CD16) antibody
      • Fc-gamma receptor IIIb (CD16) antibody
      • Fc-gamma RIII antibody
      • Fc-gamma RIII-alpha antibody
      • Fc-gamma RIIIa antibody
      • FCG 3 antibody
      • FCG3 antibody
      • FCG3A_HUMAN antibody
      • FCgammaRIIIA antibody
      • FCGR 3 antibody
      • FCGR 3A antibody
      • FCGR3 antibody
      • FCGR3A antibody
      • FCGR3A protein antibody
      • FCGRIII antibody
      • FCGRIII-2 antibody
      • FcR 10 antibody
      • FcR-10 antibody
      • FcR10 antibody
      • FcRIII antibody
      • FcRIIIa antibody
      • IGFR 3 antibody
      • IGFR3 antibody
      • IgG Fc receptor III 1 antibody
      • IgG Fc receptor III 2 antibody
      • IgG Fc receptor III-2 antibody
      • IMD20 antibody
      • immunoglobulin G Fc receptor III antibody
      • immunoglobulin G Fc receptor III-2 antibody
      • Low affinity IIIa receptor antibody
      • Low affinity immunoglobulin gamma Fc region receptor III A antibody
      • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
      • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
      • neutrophil-specific antigen NA antibody
      see all

    References for Anti-CD16 antibody [CB16] (ab1254)

    This product has been referenced in:
    • Funaro A  et al. Recognition by monoclonal antibody CB02 of a surface molecule shared by B lymphocytes and a discrete large granular lymphocyte subset with cytotoxic activity. Nat Immun Cell Growth Regul 7:106-15 (1988). Read more (PubMed: 3412357) »

    See 1 Publication for this product

    Product Wall

    I can confirm that ab12545 (clone CB16) is an IgG1 isotype.

    Thanks for contacting us regarding this mistake on our datasheet. We really appreciate the feedback. I will contact our IT department to make sure the antibody subtype is changed to ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"