Mouse monoclonal [LNK16] to CD16, prediluted (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
SpecificityThe antibody reacts with CD16 antigen that functions as a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 antigen exists in two different isoforms: CD16a is a transmembrane form (50-65 kDa) expressed on NK cells, monocytes and macrophages; CD16b is a GPI-anchored form (48 kDa) mainly expressed on neutrophils.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 20µl for 100µl human whole blood cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Flow Cytometry - CD16 antibody [LNK16] (Phycoerythrin) (ab18234)This image is courtesy of an anonymous Abreview
ab18234 staining CD16 in peripheral blood mononuclear cells by flow cytometry. 10 ul of PE conjugated antibody was added to 50 uL PMBCs (red). 10 uL of PE conjugated mouse IgG1 from eBioscience (12-4714) was used as a control (black). Cells were gated on viable cells.