Mouse monoclonal [LNK16] to CD16, prediluted (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
SpecificityThe antibody reacts with CD16 antigen that functions as a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 antigen exists in two different isoforms: CD16a is a transmembrane form (50-65 kDa) expressed on NK cells, monocytes and macrophages; CD16b is a GPI-anchored form (48 kDa) mainly expressed on neutrophils.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Used for the analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Flow Cytometry - CD16 antibody [LNK16] (Phycoerythrin) (ab18234)This image is courtesy of an anonymous Abreview
ab18234 staining CD16 in peripheral blood mononuclear cells by flow cytometry. 10 ul of PE conjugated antibody was added to 50 uL PMBCs (red). 10 uL of PE conjugated mouse IgG1 from eBioscience (12-4714) was used as a control (black). Cells were gated on viable cells.