Anti-CD16 antibody [MEM-154] - Azide free (ab46679)

Overview

  • Product nameAnti-CD16 antibody [MEM-154] - Azide free
    See all CD16 primary antibodies
  • Description
    Mouse monoclonal [MEM-154] to CD16 - Azide free
  • SpecificityThe antibody MEM-154 reacts with the epitope on CD16 antigen residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcγRIII antigen). MEM-154 recognizes a polymorphism of CD16a (NK cells form), it reacts well with granulocyte form (CD16b) of all subjects. The antibody blocks binding of human IgG to FcγRIII.
  • Tested applicationsSuitable for: WB, IP, Functional Studies, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human granulocytes

  • Positive control
    • Peripheral blood lymphocytes
  • General notesPreserved by filter sterilization

Properties

Applications

Our Abpromise guarantee covers the use of ab46679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Functional Studies
Flow Cyt
IHC-Fr
  • Application notesFlow Cyt: Use at a concentration of 5 - 10 µg/ml.
    FuncS: Use at an assay dependent dilution. The antibody blocks binding of human IgG to FcγRIII.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Use under non reducing conditions. Predicted molecular weight: 29 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
    • Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
    • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Glycosylated. Contains high mannose- and complex-type oligosaccharides.
      The soluble form is produced by a proteolytic cleavage.
    • Cellular localizationCell membrane. Secreted. Exists also as a soluble receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 16 antibody
      • CD 16a antibody
      • CD16 antibody
      • CD16a antibody
      • CD16a antigen antibody
      • CD16B antibody
      • CD16b antigen antibody
      • Fc fragment of IgG antibody
      • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
      • Fc fragment of IgG low affinity IIIa receptor antibody
      • Fc fragment of IgG receptor IIIa antibody
      • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor for antibody
      • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
      • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
      • Fc gamma R3 antibody
      • Fc gamma receptor III 2 (CD 16) antibody
      • Fc gamma receptor III A antibody
      • Fc gamma receptor IIIA antibody
      • Fc gamma receptor IIIb (CD 16) antibody
      • Fc gamma RIII alpha antibody
      • Fc gamma RIII antibody
      • Fc gamma RIII beta antibody
      • Fc gamma RIIIa antibody
      • Fc gamma RIIIb antibody
      • Fc of IgG antibody
      • Fc-gamma receptor III2 (CD 16) antibody
      • Fc-gamma receptor III2 (CD16) antibody
      • Fc-gamma receptor IIIb (CD16) antibody
      • Fc-gamma RIII antibody
      • Fc-gamma RIII-alpha antibody
      • Fc-gamma RIIIa antibody
      • FCG 3 antibody
      • FCG3 antibody
      • FCG3A_HUMAN antibody
      • FCgammaRIIIA antibody
      • FCGR 3 antibody
      • FCGR 3A antibody
      • FCGR3 antibody
      • FCGR3A antibody
      • FCGR3A protein antibody
      • FCGRIII antibody
      • FCGRIII-2 antibody
      • FcR 10 antibody
      • FcR-10 antibody
      • FcR10 antibody
      • FcRIII antibody
      • FcRIIIa antibody
      • IGFR 3 antibody
      • IGFR3 antibody
      • IgG Fc receptor III 1 antibody
      • IgG Fc receptor III 2 antibody
      • IgG Fc receptor III-2 antibody
      • IMD20 antibody
      • immunoglobulin G Fc receptor III antibody
      • immunoglobulin G Fc receptor III-2 antibody
      • Low affinity IIIa receptor antibody
      • Low affinity immunoglobulin gamma Fc region receptor III A antibody
      • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
      • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
      • neutrophil-specific antigen NA antibody
      see all

    Anti-CD16 antibody [MEM-154] - Azide free images

    • Human peripheral blood granulocytes stained with ab46679 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab46679, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood granulocytes.

    References for Anti-CD16 antibody [MEM-154] - Azide free (ab46679)

    This product has been referenced in:
    • Karagiannis P  et al. IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest 123:1457-74 (2013). IHC-Fr ; Human . Read more (PubMed: 23454746) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us. The antibody ab46679 blocks binding of human IgG to FcgammaRIII. The experiment was published in: Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding...

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    Thank you for your enquiry. I can confirm the following details which I have obtained from the laboratories: ab41899 CD32 antibody: Unfortunately, we do not have a specific protocol for the use of this product in blocking experiments, this appl...

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