Recombinant Anti-CD16 antibody [SP175] (ab183354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP175] to CD16
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD16 antibody [SP175]
See all CD16 primary antibodies -
Description
Rabbit monoclonal [SP175] to CD16 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human lung, thymus, B cell lymphoma, uterus, spleen, placenta, liver, liver hepatocellular carcinoma, lung adenocarcinoma, pancreatic adenocarcinoma and HK lymphoma tissues. Flow Cyt (Intra): Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP175 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-CD16 antibody [SP175] - BSA and Azide free (ab243925)
- Anti-CD16 antibody [SP175] - Mouse IgG2a (Chimeric) (ab308605)
- Anti-CD16 antibody [SP175] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab308606)
- Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) (ab308607)
- Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) - BSA and Azide free (ab308608)
- Alexa Fluor® 568 Anti-CD16 antibody [SP175] (ab312783)
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P | (2) |
1/100.
Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
Notes |
---|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
Target
-
Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
-
Database links
- Entrez Gene: 2214 Human
- Omim: 146740 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
-
Alternative names
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
Images
-
Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Flow cytometric analysis of Jurkat cells (Human T cell leukemia cell line from peripheral blood) labeling CD16 with ab183354 at a 1/100 dilution (green) compared to a negative control Rabbit IgG (blue).
-
Immunohistochemical analysis of paraffin embedded human lung tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human thymus tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human B cell lymphoma tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human uterus tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human liver hepatocellular carcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human lung adenocarcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human pancreatic adenocarcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin embedded human HK lymphoma tissue labeling CD16 with ab183354 at 1/100 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab183354 has been referenced in 6 publications.
- Yang W et al. Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription. J Exp Clin Cancer Res 41:67 (2022). PubMed: 35180863
- Lopez DC et al. Multi-spectral immunofluorescence evaluation of the myeloid, T cell, and natural killer cell tumor immune microenvironment in chordoma may guide immunotherapeutic strategies. Front Oncol 12:1012058 (2022). PubMed: 36338744
- Wang G et al. The pan-cancer landscape of crosstalk between epithelial-mesenchymal transition and immune evasion relevant to prognosis and immunotherapy response. NPJ Precis Oncol 5:56 (2021). PubMed: 34158591
- Zheng KF et al. PD-L1 expression and immune cells infiltration in primary tracheobronchial neoplasm. Transl Lung Cancer Res 10:4617-4630 (2021). PubMed: 35070765
- Keren L et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
- Keren L et al. A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging. Cell 174:1373-1387.e19 (2018). Mass Cytometry . PubMed: 30193111