Recombinant
RabMAb

Anti-CD16 antibody [SP175] (ab183354)

Overview

  • Product name
    Anti-CD16 antibody [SP175]
    See all CD16 primary antibodies
  • Description
    Rabbit monoclonal [SP175] to CD16
  • Tested applications
    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD16 (C terminal). The exact sequence is proprietary.
    Database link: P08637

  • Positive control
    • Human lung, thymus, B cell lymphoma, uterus, spleen, placenta, liver, liver hepatocellular carcinoma, lung adenocarcinoma, pancreatic adenocarcinoma and HK lymphoma tissues; Jurkat cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function
    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
  • Tissue specificity
    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Glycosylated. Contains high mannose- and complex-type oligosaccharides.
    The soluble form is produced by a proteolytic cleavage.
  • Cellular localization
    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 16 antibody
    • CD 16a antibody
    • CD16 antibody
    • CD16a antibody
    • CD16a antigen antibody
    • CD16B antibody
    • CD16b antigen antibody
    • Fc fragment of IgG antibody
    • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG receptor IIIa antibody
    • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor for antibody
    • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
    • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
    • Fc gamma R3 antibody
    • Fc gamma receptor III 2 (CD 16) antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIA antibody
    • Fc gamma receptor IIIb (CD 16) antibody
    • Fc gamma RIII alpha antibody
    • Fc gamma RIII antibody
    • Fc gamma RIII beta antibody
    • Fc gamma RIIIa antibody
    • Fc gamma RIIIb antibody
    • Fc of IgG antibody
    • Fc-gamma receptor III2 (CD 16) antibody
    • Fc-gamma receptor III2 (CD16) antibody
    • Fc-gamma receptor IIIb (CD16) antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-alpha antibody
    • Fc-gamma RIIIa antibody
    • FCG 3 antibody
    • FCG3 antibody
    • FCG3A_HUMAN antibody
    • FCgammaRIIIA antibody
    • FCGR 3 antibody
    • FCGR 3A antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3A protein antibody
    • FCGRIII antibody
    • FCGRIII-2 antibody
    • FcR 10 antibody
    • FcR-10 antibody
    • FcR10 antibody
    • FcRIII antibody
    • FcRIIIa antibody
    • IGFR 3 antibody
    • IGFR3 antibody
    • IgG Fc receptor III 1 antibody
    • IgG Fc receptor III 2 antibody
    • IgG Fc receptor III-2 antibody
    • IMD20 antibody
    • immunoglobulin G Fc receptor III antibody
    • immunoglobulin G Fc receptor III-2 antibody
    • Low affinity IIIa receptor antibody
    • Low affinity immunoglobulin gamma Fc region receptor III A antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
    • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
    • neutrophil-specific antigen NA antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells labeling CD16 with ab183354 at 100 dilution (green) compared to a negative control Rabbit IgG (blue).

  • Immunohistochemical analysis of paraffin embedded Human lung tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human liver hepatocellular carcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human pancreatic adenocarcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human HK lymphoma tissue labeling CD16 with ab183354 at 1/100 dilution.

References

ab183354 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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