Recombinant Anti-CD16a antibody [SP189] - C-terminal (ab227665)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP189] to CD16a - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD16a antibody [SP189] - C-terminal
See all CD16a primary antibodies -
Description
Rabbit monoclonal [SP189] to CD16a - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung, liver, thymus, spleen, placenta, B-cell lymphoma, cervical squamous cell carcinoma, colon carcinoma, HK lymphoma, stomach carcinoma, and liver hepatocellular carcinoma tissue; WB: K562 cell lysate; Flow Cyt (Intra): Jurkat cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP189 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100.
Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at |
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Flow Cyt (Intra) |
1/100.
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Notes |
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WB
1/100. Predicted molecular weight: 29 kDa. |
IHC-P
1/100. Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at |
Flow Cyt (Intra)
1/100. |
Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Involvement in disease
Immunodeficiency 20 -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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Alternative names
- CD16a antibody
- CD16a antigen antibody
- Fc-gamma RIII antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD16a with ab227665 at 1/100 (green) compared with a rabbit isotype control (blue).
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Anti-CD16a antibody [SP189] - C-terminal (ab227665) at 1/100 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Developed using the ECL technique.
Predicted band size: 29 kDa -
Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227665 has not yet been referenced specifically in any publications.