• Product nameAnti-CD161 antibody [B199.2]
    See all CD161 primary antibodies
  • Description
    Mouse monoclonal [B199.2] to CD161
  • Specificityab23624 recognises CD161 cell surface antigen, expressed by almost all NK cells and with a small subset of CD3 positive T cells.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Activated human NK cells.

  • EpitopeClone B199.2 recognises a similar epitope to the DX9 monoclonal antibody.
  • General notesMyeloma cell line: P2X63.Ag8.653


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberB199.2
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab23624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionPlays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
    • Tissue specificityExpressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
    • Sequence similaritiesContains 1 C-type lectin domain.
    • Post-translational
      N-glycosylated. Contains sialic acid residues.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-type lectin domain family 5 member B antibody
      • CD161 antibody
      • CLEC5B antibody
      • HNKR-P1a antibody
      • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
      • Killer cell lectin-like receptor subfamily B member 1 antibody
      • KLRB1 antibody
      • KLRB1_HUMAN antibody
      • Natural killer cell surface protein P1A antibody
      • NKR antibody
      • NKR P1 antibody
      • NKR-P1A antibody
      • NKRP1 antibody
      • NKRP1A antibody
      see all

    References for Anti-CD161 antibody [B199.2] (ab23624)

    This product has been referenced in:
    • Barnig C  et al. Lipoxin a4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med 5:174ra26 (2013). IHC-P ; Human . Read more (PubMed: 23447017) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil)
    Specification Tonsil
    Fixative Paraformaldehyde
    Antigen retrieval step Other
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10µg/mL · Temperature: 21°C

    Dr. James Jupp

    Verified customer

    Submitted Jan 22 2010