Anti-CD161 antibody [PK136] (ab22515)

Overview

  • Product name
    Anti-CD161 antibody [PK136]
    See all CD161 primary antibodies
  • Description
    Mouse monoclonal [PK136] to CD161
  • Tested applications
    Suitable for: Flow Cyt, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Tissue/ cell preparation (Mouse): Spleen and bone marrow cells from CE mice

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    PK136
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
Functional Studies Use a concentration of 1 - 10 µg/ml.

Target

  • Function
    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
  • Tissue specificity
    Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated. Contains sialic acid residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-type lectin domain family 5 member B antibody
    • CD161 antibody
    • CLEC5B antibody
    • HNKR-P1a antibody
    • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
    • Killer cell lectin-like receptor subfamily B member 1 antibody
    • KLRB1 antibody
    • KLRB1_HUMAN antibody
    • Natural killer cell surface protein P1A antibody
    • NKR antibody
    • NKR P1 antibody
    • NKR-P1A antibody
    • NKRP1 antibody
    • NKRP1A antibody
    see all

References for Anti-CD161 antibody [PK136] (ab22515)

ab22515 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I have been in touch with the source of this antibody and I can tell you that whilst batches are routinely tested by flow cytometry the application of in vivo depletion was added as a result of the clone being shown to ex...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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