Anti-CD161 antibody [PK136] (ab22515)


  • Product nameAnti-CD161 antibody [PK136]
    See all CD161 primary antibodies
  • Description
    Mouse monoclonal [PK136] to CD161
  • Specificityab22515 recognises CD161c. ab22515 may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by ab22515 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. ab22515 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes.
  • Tested applicationsSuitable for: Flow Cyt, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Tissue/ cell preparation (Mouse): Spleen and bone marrow cells from CE mice


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified by affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberPK136
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab22515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Functional Studies
  • Application notesFlow Cyt: Use 10µl for 106 cells, or 5 - 10 µg/ml.
    FuncS: Complement dependent lysis: Use at a concentration of 1 - 10 µg/ml.
    IP: Use at an assay dependent dilution.
    This antibody may be suitable for in vivo depletion of NK cells.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
    • Tissue specificityExpressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
    • Sequence similaritiesContains 1 C-type lectin domain.
    • Post-translational
      N-glycosylated. Contains sialic acid residues.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-type lectin domain family 5 member B antibody
      • CD161 antibody
      • CLEC5B antibody
      • HNKR-P1a antibody
      • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
      • Killer cell lectin-like receptor subfamily B member 1 antibody
      • KLRB1 antibody
      • KLRB1_HUMAN antibody
      • Natural killer cell surface protein P1A antibody
      • NKR antibody
      • NKR P1 antibody
      • NKR-P1A antibody
      • NKRP1 antibody
      • NKRP1A antibody
      see all

    References for Anti-CD161 antibody [PK136] (ab22515)

    ab22515 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I have been in touch with the source of this antibody and I can tell you that whilst batches are routinely tested by flow cytometry the application of in vivo depletion was added as a result of the clone being shown to ex...

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