Anti-CD161 antibody [PK136] (Biotin) (ab22516)

Overview

  • Product name
    Anti-CD161 antibody [PK136] (Biotin)
    See all CD161 primary antibodies
  • Description
    Mouse monoclonal [PK136] to CD161 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    This antibody recognises CD161c, and may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by ab22516 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Ab22516 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes.
  • Tested applications
    Suitable for: Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Tissue/ cell preparation (Mouse): Spleen and bone marrow cells from CE mice

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This antibody was been purified by affinity chromatography, from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    PK136
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration. In vivo depletion of NK cells and in vitro activation of NK cells.
Flow Cyt Use at an assay dependent concentration.

ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
  • Tissue specificity
    Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated. Contains sialic acid residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-type lectin domain family 5 member B antibody
    • CD161 antibody
    • CLEC5B antibody
    • HNKR-P1a antibody
    • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
    • Killer cell lectin-like receptor subfamily B member 1 antibody
    • KLRB1 antibody
    • KLRB1_HUMAN antibody
    • Natural killer cell surface protein P1A antibody
    • NKR antibody
    • NKR P1 antibody
    • NKR-P1A antibody
    • NKRP1 antibody
    • NKRP1A antibody
    see all

Anti-CD161 antibody [PK136] (Biotin) images

  • Flow cytometry of C57Bl/6 splenocytes labeling CD161 and CD3e with ab22516 at 0.25µg (right panel) or Mouse IgG2a isotype control at 0.25µg (left panel)

     

References for Anti-CD161 antibody [PK136] (Biotin) (ab22516)

ab22516 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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