The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 43 kDa).
1/100 - 1/250.
Use at an assay dependent concentration. PubMed: 23635004
Application notesIs unsuitable for Flow Cyt or IHC-P.
FunctionA SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture. (Microbial infection) Acts as a receptor for enterovirus 71.
Tissue specificityExpressed on neutrophils, monocytes and most lymphocytes.
Post-translational modificationsDisplays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation. Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
P selectin glycoprotein ligand 1 precursor antibody
P-selectin glycoprotein ligand 1 antibody
PSGL 1 antibody
Selectin P ligand antibody
Anti-CD162 antibody [EPR1967Y] images
Immunohistochemistry (Glycolmethacrylate sections) - Anti-CD162 antibody [EPR1967Y] (ab68143)Image from Blidberg K et al., Respiratory Research. 2013;14:47:. Fig 4.; doi: 10.1186/1465-9921-14-47. Published by BioMed Central
ab681432(Red) staining CD162 in Human bronchial tissue sections by Immunohistochemistry (Glycolmethacrylate sections). Tissue was embedded in Glycolmethacrylate and floated onto 0.2% ammonia solution prior to adherence to slides.Samples were incubated with primary antibody (1/200) for 1 hour. Samples were also stained with a neutrophil elastase antibody( Brown). Sections were counterstained with haematoxylin.
Key: E + x200 magnification and F = x500 magnification. * = Double stained cells.
Western blot - CD162 antibody [EPR1967Y] (ab68143)
All lanes : Anti-CD162 antibody [EPR1967Y] (ab68143) at 1/500 dilution
Lane 1 : Jurkat cell lysate at 10 µg Lane 2 : Recombinant CD162 protein at 5ng
Secondary HRP conjugated Goat anti-rabit at 1/2000 dilution