Anti-CD162 antibody [HECA-452] (ab110096)

Overview

  • Product nameAnti-CD162 antibody [HECA-452]
    See all CD162 primary antibodies
  • Description
    Rat monoclonal [HECA-452] to CD162
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Raised against a stromal preparation of human tonsils

  • Positive control
    • Human spleen or tonsil tissue. Human peripheral blood lymphocytes.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab35768-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • FunctionA SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • Tissue specificityExpressed on neutrophils, monocytes and most lymphocytes.
  • Post-translational
    modifications
    Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 162 antibody
    • CD162 antibody
    • CD162 antigen antibody
    • CLA antibody
    • Cutaneous lymphocyte associated associated antigen antibody
    • P selectin glycoprotein ligand 1 antibody
    • P selectin glycoprotein ligand 1 precursor antibody
    • P-selectin glycoprotein ligand 1 antibody
    • PSGL 1 antibody
    • PSGL-1 antibody
    • PSGL1 antibody
    • Selectin P ligand antibody
    • SELPL_HUMAN antibody
    • SELPLG antibody
    see all

Anti-CD162 antibody [HECA-452] images

  • ab110096 at 10µg/ml staining CD162 in Formalin/PFA-fixed paraffin-embedded Human spleen tissue.
  • ab110096 at 10µg/ml staining CD162 in Formalin/PFA-fixed paraffin-embedded Human tonsil tissue.

References for Anti-CD162 antibody [HECA-452] (ab110096)

ab110096 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (LPS-inflamed lung)
Permeabilization No
Specification LPS-inflamed lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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Submitted Mar 07 2016

I am very pleased to hear you would like to accept our offer and test ab110096 in ICC/IF. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"