Anti-CD162 antibody [KPL-1] (ab78188)


  • Product nameAnti-CD162 antibody [KPL-1]
    See all CD162 primary antibodies
  • Description
    Mouse monoclonal [KPL-1] to CD162
  • Tested applicationsSuitable for: Blocking, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Immunogen

    The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of CD162, previously shown to be essential for interaction with P selectin (and now shown to be essential for recognition of CD162 by L selectin).

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human aorta.
  • General notesPurified Immunoglobulin

    ab78188 completely blocks recognition of CD162 by either P selectin or by L selectin, but does not affect leukocyte recognition of E selectin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.25M% Sodium chloride, 0.02M PBS, pH 7.6,
  • Concentration information loading...
  • Primary antibody notesab78188 completely blocks recognition of CD162 by either P selectin or by L selectin, but does not affect leukocyte recognition of E selectin.
  • ClonalityMonoclonal
  • Clone numberKPL-1
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab78188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Indirect biotin streptavidin method recommended. Formalin fixed, deparaffinized sections were stained without pretreatment or microwaved for 15 minutes in 0.01 mol/L citrate buffer pH 6.0 before staining.

IHC-Fr Use at an assay dependent concentration.

Frozen sections were fixed in acetone for 10 minutes at 4 °C before staining.

Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionA SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • Tissue specificityExpressed on neutrophils, monocytes and most lymphocytes.
  • Post-translational
    Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 162 antibody
    • CD162 antibody
    • CD162 antigen antibody
    • CLA antibody
    • Cutaneous lymphocyte associated associated antigen antibody
    • P selectin glycoprotein ligand 1 antibody
    • P selectin glycoprotein ligand 1 precursor antibody
    • P-selectin glycoprotein ligand 1 antibody
    • PSGL 1 antibody
    • PSGL-1 antibody
    • PSGL1 antibody
    • Selectin P ligand antibody
    • SELPL_HUMAN antibody
    • SELPLG antibody
    see all

Anti-CD162 antibody [KPL-1] images

  • Human peripheral blood lymphocytes stained with ab78188 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab78188, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • IHC image of CD162 staining in Human Normal aorta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78188, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD162 antibody [KPL-1] (ab78188)

ab78188 has not yet been referenced specifically in any publications.

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Thank you very much for your interest in ab78188.
To our knowledge, ab78188 has not been tested in Canine. Therefore, I can offer a discount off a future purchase if you buy ab78188 now, test it in Canine and submit feedback to us in the form of a...

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Thank you very much for your interest in ab78188.

To our knowledge, ab78188 has not been tested in canine samples in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab78188 now, test it in canine samples and submit ...

Read More