Anti-CD162 antibody [TC2] (FITC) (ab88033)

Overview

  • Product name
    Anti-CD162 antibody [TC2] (FITC)
    See all CD162 primary antibodies
  • Description
    Mouse monoclonal [TC2] to CD162 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human thymocytes.

  • Positive control
    • Human peripheral blood
  • General notes

    The purified antibody(>95% by SDS-PAGE) is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab88033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • Tissue specificity
    Expressed on neutrophils, monocytes and most lymphocytes.
  • Post-translational
    modifications
    Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 162 antibody
    • CD162 antibody
    • CD162 antigen antibody
    • CLA antibody
    • Cutaneous lymphocyte associated associated antigen antibody
    • P selectin glycoprotein ligand 1 antibody
    • P selectin glycoprotein ligand 1 precursor antibody
    • P-selectin glycoprotein ligand 1 antibody
    • PSGL 1 antibody
    • PSGL-1 antibody
    • PSGL1 antibody
    • Selectin P ligand antibody
    • SELPL_HUMAN antibody
    • SELPLG antibody
    see all

Images

  • ah88033 detecting CD162 in Human peripheral blood cells by Flow cytometry

References

ab88033 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. The current lot of the anti-CD162 FITC conjugate, ab88033, lot *****, is 0.01 mg/ml. The isotype control we were looking at, ab106163, is 1.0 mg/ml, so you will want to dilute that 100-fold to match the concentration o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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