Anti-CD163 antibody [10D6], prediluted (ab74604)


  • Product nameAnti-CD163 antibody [10D6], prediluted
    See all CD163 primary antibodies
  • Description
    Mouse monoclonal [10D6] to CD163, prediluted
  • Specificityab74604 may be useful in the localization of monocytes and most macrophage types as well as aiding further study into the function and expression of CD163 antigen itself.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein encoding of domains 1-4 of the N-terminal region of human CD163.

  • EpitopeDomains 1 to 4 of the N-terminal region
  • Positive control
    • Human placenta tissue.



Our Abpromise guarantee covers the use of ab74604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionAcute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • Tissue specificityExpressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • Sequence similaritiesContains 9 SRCR domains.
  • DomainThe SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • Post-translational
    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
  • Cellular localizationSecreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • C163A_HUMAN antibody
    • CD 163 antibody
    • CD163 antibody
    • CD163 antigen antibody
    • CD163 molecule antibody
    • Hemoglobin scavenger receptor antibody
    • M130 antibody
    • M130 antigen precursor antibody
    • Macrophage associated antigen antibody
    • MM130 antibody
    • OTTHUMP00000238617 antibody
    • OTTHUMP00000238618 antibody
    • OTTHUMP00000238619 antibody
    • OTTHUMP00000238620 antibody
    • Scavenger receptor cysteine rich type 1 protein M130 antibody
    • sCD163 antibody
    • Soluble CD163 antibody
    see all

Anti-CD163 antibody [10D6], prediluted images

  • ab74604 staining CD163 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 4 minutes at 36°C; antigen retrieval was by heat mediation in EDTA pH 8.0. Samples were incubated with the prediluted primary antibody for 20 minutes at 37°C. An HRP polymer-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab74604 staining CD163 in human placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note membrane staining of monocytes and macrophages

References for Anti-CD163 antibody [10D6], prediluted (ab74604)

This product has been referenced in:
  • Bloch O  et al. Gliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages. Clin Cancer Res 19:3165-75 (2013). Read more (PubMed: 23613317) »
  • Jézéquel P  et al. Validation of tumour-associated macrophage ferritin light chain as a prognostic biomarker in node-negative breast cancer tumours: A multicentric 2004 national PHRC study. Int J Cancer : (2011). IHC ; Human . Read more (PubMed: 21898387) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH8.0
Permeabilization No
Specification Lung
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jun 24 2015

The concentration was determined to be 74 ug/ml by ELISA.