Overview

  • Product nameAnti-CD163 antibody [GHI/61]
    See all CD163 primary antibodies
  • Description
    Mouse monoclonal [GHI/61] to CD163
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    CD163 antibody was raised against spleen cells from a case of hairy cell leukemia.

  • Positive control
    • Human peripheral blood cells, Human Placenta, Human Spleen, and Human Liver tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab111250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5µg for 105-8 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAcute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • Tissue specificityExpressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • Sequence similaritiesContains 9 SRCR domains.
  • DomainThe SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • Post-translational
    modifications
    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.
  • Cellular localizationSecreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • C163A_HUMAN antibody
    • CD 163 antibody
    • CD163 antibody
    • CD163 antigen antibody
    • CD163 molecule antibody
    • Hemoglobin scavenger receptor antibody
    • M130 antibody
    • M130 antigen precursor antibody
    • Macrophage associated antigen antibody
    • MM130 antibody
    • OTTHUMP00000238617 antibody
    • OTTHUMP00000238618 antibody
    • OTTHUMP00000238619 antibody
    • OTTHUMP00000238620 antibody
    • Scavenger receptor cysteine rich type 1 protein M130 antibody
    • sCD163 antibody
    • Soluble CD163 antibody
    see all

Anti-CD163 antibody [GHI/61] images

  • Immunohistochemical staining of paraffin-embedded formalin fixed human liver using ab111250 at 20 µg/mL.

  • Negative control - immunohistochemical staining of paraffin-embedded formalin fixed human cerebral cortex using ab111250 at 20 µg/mL.

  • Formalin-Fixed, Paraffin-Embedded Human Placenta tissue stained using ab111250 at a concentration of 20 µg/ml.
  • Formalin-Fixed, Paraffin-Embedded Human Spleen tissue stained using ab111250 at a concentration of 20 µg/ml.
  • Formalin-Fixed, Paraffin-Embedded Human Liver tissue stained using ab111250 at a concentration of 20 µg/ml.
  • Staining of normal Human peripheral blood cells with 0.25 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.25 ug of ab111250 (colored histogram) followed by FITC Anti-Mouse IgG. Cells in the monocyte gate were used for analysis.

References for Anti-CD163 antibody [GHI/61] (ab111250)

This product has been referenced in:
  • Howard JD  et al. Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex. PLoS One 9:e91787 (2014). IHC-P ; Rabbit . Read more (PubMed: 24637626) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Protein Block serum free from Dako as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Antigen retrieval step Enzymatic
Sample Rabbit Tissue sections (Ear wound exudates)
Specification Ear wound exudates
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 29 2013


IF by definition refers to any antibody application that utilizes fluorescence as the detection method. Thus, technically ICC and IF are one in the same, and you could argue that IHC is also a form of IF.

For the purposes of searching for...

Read More

Muchas gracias por tu respuesta.

Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"