Overview

  • Product name
    Anti-CD163 antibody [OTI2G12]
    See all CD163 primary antibodies
  • Description
    Mouse monoclonal [OTI2G12] to CD163
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD163.
    Database link: Q86VB7

  • Positive control
    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY CD163 cDNA. IHC-P: Human liver tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY CD163.
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 2G12 to OTI2G12, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab156769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 125 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • Function
    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • Tissue specificity
    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • Sequence similarities
    Contains 9 SRCR domains.
  • Domain
    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • Post-translational
    modifications
    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.
  • Cellular localization
    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • C163A_HUMAN antibody
    • CD 163 antibody
    • CD163 antibody
    • CD163 antigen antibody
    • CD163 molecule antibody
    • Hemoglobin scavenger receptor antibody
    • M130 antibody
    • M130 antigen precursor antibody
    • Macrophage associated antigen antibody
    • MM130 antibody
    • OTTHUMP00000238617 antibody
    • OTTHUMP00000238618 antibody
    • OTTHUMP00000238619 antibody
    • OTTHUMP00000238620 antibody
    • SCARI1 antibody
    • Scavenger receptor cysteine rich type 1 protein M130 antibody
    • sCD163 antibody
    • Soluble CD163 antibody
    see all

Images

  • Paraffin-embedded human liver tissue stained for CD163 with ab156769 (1/150 dilution) in immunohistochemical analysis.

  • Sample: Human tissue lysate - whole (Skeletal muscle (Gastrocnemius).
    Specification: Skeletal muscle (Gastrocnemius).
    Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 25°C.

    ab156769 used at a 1/1000 dilution.

    A Goat Anti-Mouse Alexa Fluor® 488 was used as the secondary at a 1/5000 dilution.

    CON = Healthy patient known to express basal levels of macrophages.
    POS = Patient of interest Non specific bands more faint than band of interest in POS.

    See Abreview

  • ab156769 staining CD163 in Human skeletal muscle (Gastrocnemius) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-mouse polyclonal was used as the secondary antibody (1/200).

    See Abreview

  • Immunofluorescent analysis of COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY CD163 labeling CD163 with ab156769 at 1/100 dilution.

  • All lanes : Anti-CD163 antibody [OTI2G12] (ab156769) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY CD163 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 125 kDa



    HEK-293T cell lysates were generated from transient transfection of the cDNA clone (RC212446)

References

This product has been referenced in:
  • Tian W  et al. A prognostic risk model for patients with triple negative breast cancer based on stromal natural killer cells, tumor-associated macrophages and growth-arrest specific protein 6. Cancer Sci 107:882-9 (2016). IHC-P ; Human . Read more (PubMed: 27145494) »
  • Ha DM  et al. Transforming growth factor-beta 1 produced by vascular smooth muscle cells predicts fibrosis in the gastrocnemius of patients with peripheral artery disease. J Transl Med 14:39 (2016). IF ; Human . Read more (PubMed: 26847457) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin (epidermis to SQ fat or muscle))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Invitrogen eBioscience IHC antigen retrieval solution, low pH, 10x
Permeabilization
Yes - H2O2
Specification
Skin (epidermis to SQ fat or muscle)
Blocking step
ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% neutral buffered saline
Username

Abcam user community

Verified customer

Submitted Feb 15 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
Permeabilization
No
Specification
Human liver
Blocking step
Dako flex peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Permeabilization
No
Fixative
Methacarn
Username

Abcam user community

Verified customer

Submitted Jul 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris (pH8) Cit (pH) does not work
Sample
Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Permeabilization
No
Fixative
Methacarn
Username

Abcam user community

Verified customer

Submitted Jul 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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