Recombinant
RabMAb

Anti-CD163L1 antibody [EPR6539] (ab126756)

Overview

  • Product name
    Anti-CD163L1 antibody [EPR6539]
    See all CD163L1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6539] to CD163L1
  • Tested applications
    Suitable for: IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD163L1 aa 1400 to the C-terminus (intracellular).

  • Positive control
    • Human spleen and uterus tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR6539
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified, use 1/100 - 1/250.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Tissue specificity
      Isoform 1 is highly expressed in the spleen, lymph nodes, thymus, and fetal liver and weakly expressed in bone marrow and no expression was found in peripheral blood leukocytes. Isoform 1 expression is restricted to the monocyte and macrophage cell lines. Isoform 2 is only expressed in spleen.
    • Sequence similarities
      Contains 12 SRCR domains.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B430307C05 antibody
      • C163B_HUMAN antibody
      • CD163 antigen B antibody
      • CD163 antigen-like 1 antibody
      • CD163 molecule-like 1 antibody
      • CD163b antibody
      • CD163b antigen antibody
      • CD163L1 antibody
      • CD164L1 antibody
      • E430002D04Rik antibody
      • M160 antibody
      • RGD1307378 antibody
      • Scart1 antibody
      • Scavenger receptor cysteine-rich type 1 protein M160 antibody
      see all

    Images

    • Immunohistochemical staining of paraffin embedded human stomach with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded human spleen with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Unpurified ab126756, at 1/100 dilution, staining CD163L1 in paraffin-embedded Human spleen tissue by Immunohistochemsitry.

    • Unpurified ab126756, at 1/100 dilution, staining CD163L1 in paraffin-embedded Human uterus tissue by Immunohistochemistry.

    • Unpurified ab126756 showing positive staining in Normal tonsil macrophages tissue.

    • Unpurified ab126756 showing positive staining in Normal liver macrophages tissue.

    • Unpurified ab126756 showing positive staining in Normal lung macrophages tissue.

    • Unpurified ab126756 showing negative staining in Normal placenta tissue.

    • Unpurified ab126756 showing positive staining in Normal stomach macrophages tissue.

    References

    This product has been referenced in:
    • Hu ZQ  et al. Overexpression of semaphorin 3A promotes tumor progression and predicts poor prognosis in hepatocellular carcinoma after curative resection. Oncotarget 7:51733-51746 (2016). IHC-P ; Human . Read more (PubMed: 27351132) »
    • Stodden GR  et al. Loss of Cdh1 and Trp53 in the uterus induces chronic inflammation with modification of tumor microenvironment. Oncogene N/A:N/A (2014). Read more (PubMed: 24998851) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (metastatic melanoma)
    Permeabilization
    No
    Specification
    metastatic melanoma
    Blocking step
    (agent) for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Sep 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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