Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD166.
WB: SH-SY5Y, HuT-78, HT-1080 and Daudi cell lysates. Mouse and rat brain tissue lysates.
IHC-P: Human liver and prostatic adenocarcinoma tissues.
ICC/IF: THP-1 cells.
Flow Cyt: HuT-78 cells.
IP: SH-SY5Y cells.
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
1/90. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/250.
FunctionCell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Tissue specificitySpleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD166 with purified ab109215 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/Immunofluorescence analysis of THP-1 (human acute monocytic leukemia) cells labelling CD166 (green) with purified ab109215 at 1/250. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215)
All lanes : Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Flow Cytometry analysis of HuT-78 cells labelling CD166 with purified ab109215 at 1/90 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
ab109215 (purified) at 1/30 immunoprecipitating CD166 in SH-SY5Y cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
References for Anti-CD166 antibody [EPR2759(2)] (ab109215)
This product has been referenced in:
Zhou RP et al. Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis. Mol Med Rep11:4489-95 (2015).
Read more (PubMed: 25634525) »
Zhao Y et al. Activation of bone marrow-derived mesenchymal stromal cells-a new mechanism of defocused low-energy shock wave in regenerative medicine. Cytotherapy15:1449-57 (2013).
Read more (PubMed: 24199590) »