Anti-CD168 antibody - C-terminal (ab170527)

Overview

  • Product name
    Anti-CD168 antibody - C-terminal
    See all CD168 primary antibodies
  • Description
    Rabbit polyclonal to CD168 - C-terminal
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD168 aa 675-705 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O75330

  • Positive control
    • Ramos cell line lysates; Human breast carcinoma tissue; Ramos cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 84 kDa.

Target

  • Function
    Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
  • Tissue specificity
    Expressed in breast cancer cell lines and in normal breast tissue.
  • Cellular localization
    Cell surface. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD168 antibody
    • CD168 antigen antibody
    • HMMR antibody
    • HMMR_HUMAN antibody
    • Hyaluronan mediated motility receptor antibody
    • Hyaluronan-mediated motility receptor (RHAMM) antibody
    • IHABP antibody
    • Intracellular hyaluronic acid-binding protein antibody
    • MGC119494 antibody
    • MGC119495 antibody
    • OTTHUMP00000196920 antibody
    • Receptor for hyaluronan-mediated motility antibody
    • RHAMM antibody
    see all

Images

  • Flow cytometric analysis of Ramos cells (right histogram) labeling CD168 with ab170527 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human breast carcinoma tissue labeling CD168 with ab170527 at 1/50 dilution. Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection. 

  • Anti-CD168 antibody - C-terminal (ab170527) at 1/100 dilution + Ramos cell line lysates at 35 µg

    Predicted band size : 84 kDa

References

ab170527 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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