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Synthetic peptide within Human CD168 aa 675-705 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: O75330
Our Abpromise guarantee covers the use of ab170527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 84 kDa.|
Flow cytometric analysis of Ramos cells (right histogram) labeling CD168 with ab170527 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human breast carcinoma tissue labeling CD168 with ab170527 at 1/50 dilution. Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
ab170527 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"