Overview

  • Product nameAnti-CD168 antibody [EPR4054]
    See all CD168 primary antibodies
  • Description
    Rabbit monoclonal [EPR4054] to CD168
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD168 aa 1-100.

  • Positive control
    • Lysates of: MDA-MB-435, LnCaP, T47-D Human testis tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. antigen retrieval is recommended
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionInvolved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
    • Tissue specificityExpressed in breast cancer cell lines and in normal breast tissue.
    • Cellular localizationCell surface. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD168 antibody
      • CD168 antigen antibody
      • HMMR antibody
      • HMMR_HUMAN antibody
      • Hyaluronan mediated motility receptor antibody
      • Hyaluronan-mediated motility receptor (RHAMM) antibody
      • IHABP antibody
      • Intracellular hyaluronic acid-binding protein antibody
      • MGC119494 antibody
      • MGC119495 antibody
      • OTTHUMP00000196920 antibody
      • Receptor for hyaluronan-mediated motility antibody
      • RHAMM antibody
      see all

    Anti-CD168 antibody [EPR4054] images

    • Immunohistochemical analysis of paraffin-embedded human testis tissue using ab124729 at 1/50 - 1/100 dilution
    • All lanes : Anti-CD168 antibody [EPR4054] (ab124729)

      Lane 1 : MDA-MB-435 cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : T47-D cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD168 antibody [EPR4054] (ab124729)

    This product has been referenced in:
    • Coulson-Thomas VJ  et al. Umbilical Cord Mesenchymal Stem Cells Suppress Host Rejection: THE ROLE OF THE GLYCOCALYX. J Biol Chem 289:23465-81 (2014). ICC/IF ; Human . Read more (PubMed: 24986866) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 35 µg
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Sample Dog Cell lysate - whole cell (canine mammay tumor cells)
    Specification canine mammay tumor cells
    Treatment siRNA against CD168 for 24 hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Saitou Teruyoshi

    Verified customer

    Submitted Jan 20 2015

    For ab124729 lot GR82264-8, the concentration is 1.797 mg/ml.

    The current lot we have for sale for ab108339 is lot GR106937-4 and that is 0.193 mg/ml.

    Thank you for contacting Abcam earlier today.

    Please find attached the IHC-P protocol for both ab108339 & ab124729.

    If there is anything else I can help you with, please let me know.

    Thank you for your email.



    I am glad to hear that the free of charge replacement worked as expected and you have obtained satisfactory results.



    If you wish, you could submit your results as feedback using our Ab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"