Overview

  • Product nameAnti-CD168 antibody [EPR4055]
    See all CD168 primary antibodies
  • Description
    Rabbit monoclonal [EPR4055] to CD168
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human CD168.

  • Positive control
    • T47-D MCF-7, SKBR-3 and LnCaP cell lysate Paraffin-embedded human breast carcinoma tissue Paraffin-embedded human testis tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionInvolved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
    • Tissue specificityExpressed in breast cancer cell lines and in normal breast tissue.
    • Cellular localizationCell surface. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD168 antibody
      • CD168 antigen antibody
      • HMMR antibody
      • HMMR_HUMAN antibody
      • Hyaluronan mediated motility receptor antibody
      • Hyaluronan-mediated motility receptor (RHAMM) antibody
      • IHABP antibody
      • Intracellular hyaluronic acid-binding protein antibody
      • MGC119494 antibody
      • MGC119495 antibody
      • OTTHUMP00000196920 antibody
      • Receptor for hyaluronan-mediated motility antibody
      • RHAMM antibody
      see all

    Anti-CD168 antibody [EPR4055] images

    • All lanes : Anti-CD168 antibody [EPR4055] (ab108339) at 1/1000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
    • ab108339, at 1/100, staining CD168 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
    • ab108339, at 1/100, staining CD168 in paraffin-embedded human testis tissue by Immunohistochemistry.
    • ab108339 showing positive staining in Normal thymus tissue.

    • ab108339 showing positive staining in Normal stomach tissue.

    • ab108339 showing negative staining in Normal brain tissue.

    • ab108339 showing positive staining in Colonic adenocarcinoma tissue.

    • ab108339 showing negative staining in Normal liver tissue.

    • ab108339 showing positive staining in Normal tonsil tissue.

    References for Anti-CD168 antibody [EPR4055] (ab108339)

    This product has been referenced in:
    • Wang K & Zhang T Prognostic significance of CD168 overexpression in colorectal cancer. Oncol Lett 12:2555-2559 (2016). IHC ; Human . Read more (PubMed: 27698827) »
    • Picaud L  et al. Evaluation of the effects of hyaluronic acid-carboxymethyl cellulose barrier on ovarian tumor progression. J Ovarian Res 7:40 (2014). WB ; Human . Read more (PubMed: 24739440) »

    See all 2 Publications for this product

    Product Wall

    For ab124729 lot GR82264-8, the concentration is 1.797 mg/ml.

    The current lot we have for sale for ab108339 is lot GR106937-4 and that is 0.193 mg/ml.

    There should not be concerns about permeabilizing cells because as soon as they are fixed with parafomraldehyde, they are essentially permeabilized. The CD168 antigen is a non-integral cell surface hyaluronan receptor that is readily accessable to the ...

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    A link below is our Flow Cytometry protocol, which we use routinely for in-house testing.
    http://www.epitomics.com/products/support/fc
    Our protocol is for intracellular staining and we always fix the cells with 2% paraformaldehyde.

    Thank you for your email.
    I can confirm that ab108339 (Anti-CD168 antibody) would recognize all 4 isoforms of CD168/RHAMM.
    If you need any further assistance in the future, please do not hesitate to contact me.

    Thank you for contacting Abcam earlier today.

    Please find attached the IHC-P protocol for both ab108339 & ab124729.

    If there is anything else I can help you with, please let me know.

    Thank you for contacting us.
    The target CD168 is a cell surface protein. There no need for cell permeabilization and fixation (do not wait longer than an hour). Cell fixatiion is advised if there is a wait longer than an hour between analysis. Avo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"