Anti-CD16b antibody [MM0272-5L11] (ab89207)

Overview

  • Product name
    Anti-CD16b antibody [MM0272-5L11]
  • Description
    Mouse monoclonal [MM0272-5L11] to CD16b
  • Specificity
    ab89207 does not detect CD16a.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant CD16b extracellular domain.

  • General notes
    Reconstituted antibody should be aliquoted and can be stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Properties

  • Form
    Lyophilised:Reconstitute the antibody with 200 µl sterile PBS to give a final concentration of 0.5mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at -80°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    MM0272-5L11
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/50 - 1/100. ab18414-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • CD16 antibody
    • CD16b antibody
    • CD16b antigen antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIII antibody
    • Fc gamma RIII beta antibody
    • Fc gamma RIIIb antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-beta antibody
    • Fc-gamma RIIIb antibody
    • FCG3 antibody
    • FCG3B_HUMAN antibody
    • FCGR3 antibody
    • FCGR3B antibody
    • FcR 10 antibody
    • FcR-10 antibody
    • FcR10 antibody
    • FcRIII antibody
    • FcRIIIb antibody
    • IGFR3 antibody
    • IgG Fc receptor III 1 antibody
    • IgG Fc receptor III-1 antibody
    • Low affinity immunoglobulin gamma Fc region receptor III B antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-B antibody
    see all

References

ab89207 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Both PMN and NK cells were used.

ab89207 only picks up CD16b on PMN, but not CD16a on NK cells.



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I have heard back regarding ab89207:
This clone was tested by flow cytometry to only pick up CD16b+ cells, but not CD16a+ cells.

I am still waiting for more information about ab130057.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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