Overview

  • Product nameAnti-CD16+CD32 antibody [93]
    See all CD16+CD32 primary antibodies
  • Description
    Rat monoclonal [93] to CD16+CD32
  • Specificityab25235 recognises CD16/Fc gamma II and CD32/Fc gamma III receptors.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25235 reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
  • Positive control
    • ICC/IF: RAW246.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 3µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Blocking Use 2µg for 106 cells.

ab25235 can be used for blocking of Fc gamma receptors.

Target

  • RelevanceFunction: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
  • Cellular localizationCD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
  • Database links
  • Alternative names
    • CD16a antibody
    • CD16b antibody
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma-RIIa antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRII-a antibody
    • FcRIII antibody
    • IgG Fc receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    see all

Anti-CD16+CD32 antibody [93] images

  • ICC/IF image of ab25235 stained RAW246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25235, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab98420, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry analysis of BALB/c mouse splenocytes cells labeling CD16/32 with ab25235 at 1 μg/106 cells dilution. A Rat Anti-Mouse CD19-FITC was used as the secondary antibody.

References for Anti-CD16+CD32 antibody [93] (ab25235)

ab25235 has not yet been referenced specifically in any publications.

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