• Product nameAnti-CD16+CD32 antibody [93] (PE/Cy7 ®)
    See all CD16+CD32 primary antibodies
  • Description
    Rat monoclonal [93] to CD16+CD32 (PE/Cy7 ®)
  • ConjugationPE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Specificityab25495 recognises CD16/Fc gamma II and CD32/Fc gamma III receptors.
  • Tested applicationsSuitable for: Flow Cyt, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25495 recognises a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.



Our Abpromise guarantee covers the use of ab25459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells. ab154461-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration. Blocks Fc gamma receptors.


  • RelevanceIn mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes. The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.
  • Cellular localizationCD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
  • Database links
  • Alternative names
    • CD16a antibody
    • CD16b antibody
    • CD32A antibody
    • CD32B antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRIII antibody
    see all

References for Anti-CD16+CD32 antibody [93] (PE/Cy7 ®) (ab25459)

This product has been referenced in:

See all 2 Publications for this product

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