The details of the immunogen for this antibody are not available.
Epitopeab25495 recognises a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Storage instructionsShipped at 4°C. Store at +4°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab154461-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Blocks Fc gamma receptors.
RelevanceFunction: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets.
Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Cellular localizationCD32: Type I membrane protein.
CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
Flow cytometry analysis of BALB/c mouse splenocytes labeling CD16 + CD32 with ab25459 at 0.1 µg/106cells and CD19 with Rat Anti-Mouse CD19-PE.
References for Anti-CD16+CD32 antibody  (PE/Cy7 ®) (ab25459)
This product has been referenced in:
Mercher T et al. The OTT-MAL fusion oncogene activates RBPJ-mediated transcription and induces acute megakaryoblastic leukemia in a knockin mouse model. J Clin Invest119:852-64 (2009).
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