Overview

  • Product nameAnti-CD16+CD32 antibody [93] (PE/Cy7 ®)
    See all CD16+CD32 primary antibodies
  • Description
    Rat monoclonal [93] to CD16+CD32 (PE/Cy7 ®)
  • ConjugationPE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Specificityab25495 recognises CD16/Fc gamma II and CD32/Fc gamma III receptors.
  • Tested applicationsSuitable for: Flow Cyt, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25495 recognises a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab25459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells. ab154461-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration. Blocks Fc gamma receptors.

Target

  • RelevanceFunction: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
  • Cellular localizationCD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
  • Database links
  • Alternative names
    • CD16a antibody
    • CD16b antibody
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma-RIIa antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRII-a antibody
    • FcRIII antibody
    • IgG Fc receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    see all

Anti-CD16+CD32 antibody [93] (PE/Cy7 ®) images

  • Flow cytometry analysis of BALB/c mouse splenocytes labeling CD16 + CD32 with ab25459 at 0.1 µg/106cells and CD19 with Rat Anti-Mouse CD19-PE.

References for Anti-CD16+CD32 antibody [93] (PE/Cy7 ®) (ab25459)

This product has been referenced in:

See all 2 Publications for this product

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