Anti-CD16+CD32 antibody [FCR 4G8] (ab24187)

Overview

  • Product nameAnti-CD16+CD32 antibody [FCR 4G8]
    See all CD16+CD32 primary antibodies
  • Description
    Rat monoclonal [FCR 4G8] to CD16+CD32
  • SpecificityThis antibody was specifically recognises mouse CD16 and CD32.
  • Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: PU5 1.8 IOE7 Balb/c mouse cell line.

  • Positive control
    • Mouse peripheral blood lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab24187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/25 - 1/50. Ab37371-Rat monoclonal IgG, is suitable for use as an isotype control with this antibody.

ab37371-Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceIn mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes. The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.
  • Cellular localizationCD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
  • Database links
  • Alternative names
    • CD16a antibody
    • CD16b antibody
    • CD32A antibody
    • CD32B antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRIII antibody
    see all

References for Anti-CD16+CD32 antibody [FCR 4G8] (ab24187)

ab24187 has not yet been referenced specifically in any publications.

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