The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/10.
Neat - 1/10. Use 10µl to label 106 cells in 100µl.
ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
RelevanceFunction: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets.
Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Cellular localizationCD32: Type I membrane protein.
CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
Fc fragment of IgG low affinity IIa receptor antibody
Fc fragment of IgG low affinity IIb receptor antibody
Fc fragment of IgG low affinity IIIa receptor antibody
Fc fragment of IgG low affinity IIIb receptor antibody
Fc gamma receptor III A antibody
Fc gamma receptor IIIb antibody
Fc gamma RIIa antibody
Fc gamma RIIb antibody
Fc gamma RIII beta antibody
Fc-gamma RII-a antibody
IgG Fc receptor II-a antibody
Low affinity immunoglobulin gamma Fc region receptor II-a antibody
References for Anti-CD16+CD32 antibody [FCR 4G8] (Phycoerythrin) (ab24537)
This product has been referenced in:
Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res37:1374-82 (2013).
Read more (PubMed: 23806234) »