Anti-CD16+CD32 antibody [FCR 4G8] (Phycoerythrin) (ab24537)

Overview

  • Product name
    Anti-CD16+CD32 antibody [FCR 4G8] (Phycoerythrin)
    See all CD16+CD32 primary antibodies
  • Description
    Rat monoclonal [FCR 4G8] to CD16+CD32 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    PU5 1.8 IOE7 Balb/c cell line (Mouse)

  • General notes


    ab24537 recognises an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    ab24537 recognises an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32).
  • Clonality
    Monoclonal
  • Clone number
    FCR 4G8
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/10.

Neat - 1/10. Use 10µl to label 106 cells in 100µl.





ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
  • Cellular localization
    CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
  • Database links
  • Alternative names
    • CD16a antibody
    • CD16b antibody
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma-RIIa antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRII-a antibody
    • FcRIII antibody
    • IgG Fc receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    see all

References for Anti-CD16+CD32 antibody [FCR 4G8] (Phycoerythrin) (ab24537)

This product has been referenced in:
  • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23806234) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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