The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/10.
Neat - 1/10. Use 10µl to label 106 cells in 100µl.
ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
RelevanceIn mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes. The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.
Cellular localizationCD32: Type I membrane protein.
CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
Fc fragment of IgG low affinity IIa receptor antibody
Fc fragment of IgG low affinity IIb receptor antibody
Fc fragment of IgG low affinity IIIa receptor antibody
Fc fragment of IgG low affinity IIIb receptor antibody
Fc gamma receptor III A antibody
Fc gamma receptor IIIb antibody
Fc gamma RIIa antibody
Fc gamma RIIb antibody
Fc gamma RIII beta antibody
References for Anti-CD16+CD32 antibody [FCR 4G8] (Phycoerythrin) (ab24537)
This product has been referenced in:
Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res37:1374-82 (2013).
Read more (PubMed: 23806234) »