The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets.
Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
CD32: Type I membrane protein.
CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.