Overview

  • Product name
    Anti-CD177 antibody [MEM-166]
    See all CD177 primary antibodies
  • Description
    Mouse monoclonal [MEM-166] to CD177
  • Host species
    Mouse
  • Specificity
    CD177 antigen (human)
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey, Rhesus monkey
  • Immunogen

    Human granulocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from TCS
  • Clonality
    Monoclonal
  • Clone number
    MEM-166
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration. PubMed: 23599270
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.

Target

  • Tissue specificity
    Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
  • Sequence similarities
    Contains 2 UPAR/Ly6 domains.
  • Post-translational
    modifications
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD177 antibody
    • CD177 antigen antibody
    • CD177 molecule antibody
    • CD177_HUMAN antibody
    • HNA-2a antibody
    • HNA2A antibody
    • Human neutrophil alloantigen 2a antibody
    • NB1 antibody
    • NB1 glycoprotein antibody
    • NB1 GP antibody
    • Polycythemia rubra vera 1 antibody
    • Polycythemia rubra vera protein 1 antibody
    • PRV-1 antibody
    • PRV1 antibody
    see all

Images

  • ab8092 at 1/200 staining human eripheral blood derived CD34+ cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG was used as the secondary. The cells wered counter stained with DAPI.

    See Abreview

References

This product has been referenced in:
  • Hu N  et al. Differential Expression of Granulopoiesis Related Genes in Neutrophil Subsets Distinguished by Membrane Expression of CD177. PLoS One 9:e99671 (2014). WB, Flow Cyt ; Human . Read more (PubMed: 24926686) »
  • Stepensky P  et al. The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy. Blood 121:5078-87 (2013). IHC-P ; Human . Read more (PubMed: 23599270) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

According to our datasheet, the only thing which needs consideration is the fact that this antibody will detect under non-reducing conditions only. This would mean that you would have to leave out the reducing agen...

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Thank you for contacting us regarding testing of AB8092 on rabbit tissues.

I have had the opportunity to BLAST the immunogen against rabbit on both Uniprot and NCBI Unfortunately, the best homologies that I have found are have identities of ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Peripheral blood derived CD34+ cells)
Specification
Peripheral blood derived CD34+ cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 26 2007

Thank you for your enquiry. I am sorry to hear that you have been having difficulty with this antibody. You have tried many of the suggestions that I would make such as applying this antibody at 4oC overnight using BSA as a blocking agent. You are a...

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The epitope recognised by this mouse monoclonal antibody has not been mapped. It is supplied at a concentration of 1 mg/ml so I'd recommend a 1:1000 starting dilution for WB. The antibody has been shown to be suitbale for use in WB, IP and flow cytomet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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