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Our Abpromise guarantee covers the use of ab8092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 23599270|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Use under non reducing condition.|
ab8092 at 1/200 staining human eripheral blood derived CD34+ cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG was used as the secondary. The cells wered counter stained with DAPI.