APC Anti-CD177 antibody [MEM-166] (ab77230)
Key features and details
- APC Mouse monoclonal [MEM-166] to CD177
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD177 antibody [MEM-166]
See all CD177 primary antibodies -
Description
APC Mouse monoclonal [MEM-166] to CD177 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD177. Human granulocytes
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Positive control
- Human blood cells.
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General notes
Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% (w/v) high-grade protease free BSA -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
MEM-166 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl for 106 cells or 100µl of whole blood. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl for 106 cells or 100µl of whole blood. |
Target
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Tissue specificity
Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57126 Human
- Omim: 162860 Human
- SwissProt: Q8N6Q3 Human
- Unigene: 232165 Human
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Alternative names
- CD177 antibody
- CD177 antigen antibody
- CD177 molecule antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab77230 has been referenced in 3 publications.
- Liu H et al. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria. Exp Ther Med 15:2525-2532 (2018). PubMed: 29467851
- Meyerson HJ et al. CD177 expression on neutrophils: in search of a clonal assay for myeloid neoplasia by flow cytometry. Am J Clin Pathol 140:658-69 (2013). PubMed: 24124144
- Zhou J et al. CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses. J Exp Med 207:1951-66 (2010). Flow Cyt ; Human . PubMed: 20733031