The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 10µl for 106 cells or 100µl of whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityHighly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
Sequence similaritiesContains 2 UPAR/Ly6 domains.
Post-translational modificationsN-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
References for Anti-CD177 antibody [MEM-166] (Allophycocyanin) (ab77230)
This product has been referenced in:
Zhou J et al. CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses. J Exp Med207:1951-66 (2010).
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