Overview

  • Product nameAnti-CD177 antibody [MEM-166] (Allophycocyanin)
    See all CD177 primary antibodies
  • Description
    Mouse monoclonal [MEM-166] to CD177 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Human granulocytes

  • Positive control
    • Human blood cells.
  • General notes

    Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% (w/v) high-grade protease free BSA, PBS
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesThe conjugate is purified by size-exclusion chromatography and adjusted for direct use.
  • ClonalityMonoclonal
  • Clone numberMEM-166
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl for 106 cells or 100µl of whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityHighly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
    • Sequence similaritiesContains 2 UPAR/Ly6 domains.
    • Post-translational
      modifications
      N-glycosylated.
      A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD177 antibody
      • CD177 antigen antibody
      • CD177 molecule antibody
      • CD177_HUMAN antibody
      • HNA-2a antibody
      • HNA2A antibody
      • Human neutrophil alloantigen 2a antibody
      • NB1 antibody
      • NB1 glycoprotein antibody
      • NB1 GP antibody
      • Polycythemia rubra vera 1 antibody
      • Polycythemia rubra vera protein 1 antibody
      • PRV-1 antibody
      • PRV1 antibody
      see all

    References for Anti-CD177 antibody [MEM-166] (Allophycocyanin) (ab77230)

    This product has been referenced in:
    • Zhou J  et al. CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses. J Exp Med 207:1951-66 (2010). Flow Cyt ; Human . Read more (PubMed: 20733031) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab77230.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"