Overview

  • Product nameAnti-CD177 antibody [MEM-166] (FITC)
    See all CD177 primary antibodies
  • Description
    Mouse monoclonal [MEM-166] to CD177 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Human granulocytes.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesSize exclusion chromatography.
  • ClonalityMonoclonal
  • Clone numberMEM-166
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells. or 100 µl of whole blood. (The antibody should be used undiluted with the samples).



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Tissue specificityHighly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
  • Sequence similaritiesContains 2 UPAR/Ly6 domains.
  • Post-translational
    modifications
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD177 antibody
    • CD177 antigen antibody
    • CD177 molecule antibody
    • CD177_HUMAN antibody
    • HNA-2a antibody
    • HNA2A antibody
    • Human neutrophil alloantigen 2a antibody
    • NB1 antibody
    • NB1 glycoprotein antibody
    • NB1 GP antibody
    • Polycythemia rubra vera 1 antibody
    • Polycythemia rubra vera protein 1 antibody
    • PRV-1 antibody
    • PRV1 antibody
    see all

Anti-CD177 antibody [MEM-166] (FITC) images

  • Surface staining of human peripheral blood cells with anti-CD177.

    ab26013 was used at a recommended concentration of 1 μg/mL.

References for Anti-CD177 antibody [MEM-166] (FITC) (ab26013)

This product has been referenced in:
  • Hu N  et al. Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration. Arthritis Res Ther 13:R201 (2011). Flow Cyt ; Human . Read more (PubMed: 22152684) »

See 1 Publication for this product

Product Wall

Thank you very much for your phone call! I can confirm that the total volume of the product is 2ml which means the product should be used undiluted in Flow Cytometry. I hope this information will be helpful. Please do not hesitate to contact me i...

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Thank you for your enquiry. Further to correspondence with the source of this enquiry I can tell you that whilst the immunogen of ab26013 was isolated human granulocytes the expression of CD177 has been described in monocytes. I would therefore pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"