PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)
Key features and details
- PE Mouse monoclonal [MEM-166] to CD177, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD177 antibody [MEM-166], prediluted
See all CD177 primary antibodies -
Description
PE Mouse monoclonal [MEM-166] to CD177, prediluted -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD177. Human granulocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. -
Clonality
Monoclonal -
Clone number
MEM-166 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Use 20 μl per 100 μl of whole blood or 106 cells in a suspension |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Use 20 μl per 100 μl of whole blood or 106 cells in a suspension |
Target
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Tissue specificity
Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57126 Human
- Omim: 162860 Human
- SwissProt: Q8N6Q3 Human
- Unigene: 232165 Human
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Alternative names
- CD177 antibody
- CD177 antigen antibody
- CD177 molecule antibody
see all
Images
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Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab69777 (20 μl reagent / 100 μl of peripheral whole blood).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab69777 (20 μl reagent / 100 μl of peripheral whole blood).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab69777 has been referenced in 1 publication.
- Amirbeagi F et al. Olfactomedin-4 autoantibodies give unusual c-ANCA staining patterns with reactivity to a subpopulation of neutrophils. J Leukoc Biol 97:181-9 (2015). PubMed: 25387833