Overview

  • Product nameAnti-CD18 antibody [3EB] (FITC)
    See all CD18 primary antibodies
  • Description
    Mouse monoclonal [3EB] to CD18 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Peripheral blood lymphocytes.
  • General notes


    ab52049 is useful for monitoring of subsets in peripheral blood; analysis of CTL mediated cytotoxicity; studies of transplantation rejection, and functional studies of cell adhesion molecules.

Properties

Applications

Our Abpromise guarantee covers the use of ab52049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl for 106 cells, or 10µl per 100µl whole blood.





ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • Sequence similaritiesBelongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Anti-CD18 antibody [3EB] (FITC) images

  • ab52049, at a concentration of 10µl for 100µl of whole blood, staining CD18 in Peripheral Blood Lymphocytes by Flow Cytometry.

References for Anti-CD18 antibody [3EB] (FITC) (ab52049)

This product has been referenced in:
  • Hosoki K  et al. Staphylococcus aureus directly activates eosinophils via platelet-activating factor receptor. J Leukoc Biol 92:333-41 (2012). Read more (PubMed: 22595142) »
  • Ito T  et al. Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets. J Exp Med 195:1507-12 (2002). Read more (PubMed: 12045249) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"