The details of the immunogen for this antibody are not available.
Peripheral blood lymphocytes.
ab52049 is useful for monitoring of subsets in peripheral blood; analysis of CTL mediated cytotoxicity; studies of transplantation rejection, and functional studies of cell adhesion molecules.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.08% Sodium Azide
Constituents: PBS, 0.2% protein carrier.
Concentration information loading...
Primary antibody notesab52049 is useful for monitoring of subsets in peripheral blood; analysis of CTL mediated cytotoxicity; studies of transplantation rejection, and functional studies of cell adhesion molecules.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells.
Use 10µl for 106 cells, or 10µl per 100µl whole blood.
ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family. Contains 1 VWFA domain.
Post-translational modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
ab52049, at a concentration of 10µl for 100µl of whole blood, staining CD18 in Peripheral Blood
Lymphocytes by Flow Cytometry.
References for Anti-CD18 antibody [3EB] (FITC) (ab52049)
This product has been referenced in:
Hosoki K et al. Staphylococcus aureus directly activates eosinophils via platelet-activating factor receptor. J Leukoc Biol92:333-41 (2012).
Read more (PubMed: 22595142) »
Ito T et al. Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets. J Exp Med195:1507-12 (2002).
Read more (PubMed: 12045249) »