The details of the immunogen for this antibody are not available.
All peripheral blood lymphocytes.
ab52051 is useful for monitoring of subsets in peripheral blood; analysis of CTL mediated cytotoxicity; studies of transplantation rejection and functional studies of cell adhesion molecules.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.08% Sodium Azide
Constituents: PBS and 0.2% protein carrier
Concentration information loading...
Primary antibody notesab52051 is useful for monitoring of subsets in peripheral blood; analysis of CTL mediated cytotoxicity; studies of transplantation rejection and functional studies of cell adhesion molecules.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells.
Use 10µl for 106 cells or 10µl per 100µl whole blood.
Use at an assay dependent concentration.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family. Contains 1 VWFA domain.
Post-translational modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Lymphocyte function associated antigen 1 (LFA1) antibody
Lymphocyte function associated antigen 1 antibody
MAC 1 antibody
Macrophage antigen 1 (mac1) beta antibody
References for Anti-CD18 antibody [3EB] (Phycoerythrin) (ab52051)
This product has been referenced in:
Ito T et al. Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets. J Exp Med195:1507-12 (2002).
Read more (PubMed: 12045249) »