The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab126030-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family. Contains 1 VWFA domain.
Post-translational modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
ab123625, at 5 µl/106 cells, staining CD18 in normal Human peripheral blood cells by Flow Cytometry. Total cells were used for analysis: lymphocyte (blue histogram), monocyte (green) and granulocyte gate (yellow).
References for Anti-CD18 antibody [GRF1] (OC515) (ab123625)
has not yet been referenced specifically in any publications.
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