Overview

  • Product name
    Anti-CD18 antibody [M18/2] (FITC)
    See all CD18 primary antibodies
  • Description
    Rat monoclonal [M18/2] to CD18 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Murine cytotoxic T cell glycoproteins

  • General notes

    The purified antibody is conjugated (from highly purified antibodies >95% by SDS-PAGE) with FITC under optimum conditions. The reagent is free of unconjugated FITC.

Properties

Applications

Our Abpromise guarantee covers the use of ab96575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 3 µg/ml. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • Involvement in disease
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Images

  • Flow Cytometry analysis of mouse splenocytes labeling CD18 with Anti-CD18 antibody [M18/2] (FITC) (ab96575).

References

This product has been referenced in:

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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