The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use neat - 20µl reagent/ 100µl of whole blood or 106 cells in suspension.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family. Contains 1 VWFA domain.
Post-translational modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Lymphocyte function associated antigen 1 (LFA1) antibody
Lymphocyte function associated antigen 1 antibody
MAC 1 antibody
Macrophage antigen 1 (mac1) beta antibody
References for Anti-CD18 antibody [MEM-148], prediluted (Allophycocyanin) (ab40911)
This product has been referenced in:
Drbal K et al. A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins. Immunobiology203:687-98 (2001).
Read more (PubMed: 11402502) »
Drbal K et al. A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood98:1561-6 (2001).
Read more (PubMed: 11520808) »