Anti-CD18 antibody [MEM-148], prediluted (Allophycocyanin) (ab40911)

Overview

  • Product nameAnti-CD18 antibody [MEM-148], prediluted (Allophycocyanin)
    See all CD18 primary antibodies
  • Description
    Mouse monoclonal [MEM-148] to CD18, prediluted (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • SpecificityThis antibody is specific for the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high affinity state of LFA-1.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human peripheral blood mononuclear cells.

  • General notes

    The conjugate was purified by size-exclusion chromatography and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab40911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use neat - 20µl reagent/ 100µl of whole blood or 106 cells in suspension.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
    • Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
    • Sequence similaritiesBelongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications
      Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 95 subunit beta antibody
      • Beta 2 (antigen CD18 (p95) antibody
      • CD 18 antibody
      • CD18 antibody
      • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
      • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
      • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
      • Complement component 3 receptor 3 and 4 subunit antibody
      • Complement receptor C3 beta subunit antibody
      • Complement receptor C3 subunit beta antibody
      • Integrin beta 2 antibody
      • Integrin beta chain beta 2 antibody
      • Integrin beta-2 (ITGB2) antibody
      • Integrin beta-2 antibody
      • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
      • ITB2_HUMAN antibody
      • ITGB2 antibody
      • LAD antibody
      • LCAMB antibody
      • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
      • Leukocyte cell adhesion molecule CD18 antibody
      • LFA 1 antibody
      • LFA1 antibody
      • Lymphocyte function associated antigen 1 (LFA1) antibody
      • Lymphocyte function associated antigen 1 antibody
      • MAC 1 antibody
      • MAC1 antibody
      • Macrophage antigen 1 (mac1) beta antibody
      • MF17 antibody
      • MFI7 antibody
      • OTTHUMP00000115278 antibody
      • OTTHUMP00000115279 antibody
      • OTTHUMP00000115280 antibody
      • OTTHUMP00000115281 antibody
      • OTTHUMP00000115282 antibody
      see all

    References for Anti-CD18 antibody [MEM-148], prediluted (Allophycocyanin) (ab40911)

    This product has been referenced in:
    • Drbal K  et al. A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins. Immunobiology 203:687-98 (2001). Read more (PubMed: 11402502) »
    • Drbal K  et al. A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood 98:1561-6 (2001). Read more (PubMed: 11520808) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"