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Leucocytes of a patient suffering from a LGL-type leukaemia.
Our Abpromise guarantee covers the use of ab46700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 85 kDa. Use under non reducing conditions. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. SDS-PAGE (6% separating gel, 4% stacking gel).|
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. This antibody is useful for studies of cell-cell and cell-matrix adhesion. It induces high-affinity conformation of LFA-1 complex.|
Overlay histogram showing peripheral blood lymphocytes stained with ab46700 (red line). The cells were incubated with the antibody (ab46700, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.