Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (ab176540)
Key features and details
- Mouse monoclonal [MEM148] to CD18 - Low endotoxin, Azide free
- Suitable for: IP, WB, Functional Studies, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free
See all CD18 primary antibodies -
Description
Mouse monoclonal [MEM148] to CD18 - Low endotoxin, Azide free -
Host species
Mouse -
Tested applications
Suitable for: IP, WB, Functional Studies, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Pig -
Immunogen
Tissue, cells or virus corresponding to Human CD18. Peripheral blood mononuclear cells.
Database link: P05107 -
Epitope
ab176540 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. -
General notes
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
Buffer is 0.2 µm filter sterilized. -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM148 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
Non-reducing conditions. |
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Functional Studies |
Use at an assay dependent concentration.
ab176540 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. |
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa. Non-reducing conditions. |
Functional Studies
Use at an assay dependent concentration. ab176540 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
Involvement in disease
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Post-translational
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3689 Human
- Omim: 600065 Human
- SwissProt: P05107 Human
- Unigene: 375957 Human
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Alternative names
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab176540 has not yet been referenced specifically in any publications.